Coating Made fromPseudotsuga menziesiiPhytosynthesized Silver Nanoparticles is Efficient AgainstStaphylococcus aureusBiofilm Formation

Abstract

In this nano era, biomaterials associated infection is a serious problem in the biomedical arena. The race between microbial adhesion and tissue integration becomes a major cause of concern, during the implantation process. Microbial adhesion further gives rise to biofilm formation which finally leads to implant failure. We have therefore designed a strategy to fight effectively against the encroachment of Staphylococcus aureus biofilm, which is chiefly responsible for majority of biomaterials associated infections. Silver nanoparticles have been synthesized for the purpose using foliage needles of the plant Pseudotsuga menziesii, our Christmas tree. Thereafter the nanoparticles were dispersed in chitosan, a biopolymer matrix and a bionanocomposite, self-sterilizing coating biomaterial was developed. The silver nanoparticles produced, the bionanocomposite developed, and the coating over medical implant, have been characterized through various techniques. The efficacy of the silver/chitosan bionanocomposite, against S. aureus biofilm has been studied here, after being coated over medical implant. It was found that coating of medical implants with this material can definitely restrict bacterial adhesion and their subsequent biofilm formation. This biomaterial was found to be blood and biocompatible at specific levels through testing.