Preparation and biological evaluation of a carrier free 90yttrium labelled porphyrin as a possible agent for targeted therapy of tumor

Abstract

In this research article, 5,10,15,20-tetrakis(phenyl)porphyrin (H2TPP) was produced and characterized. Then, radiolabeling of H2TPP was performed using the carrier free Y-90 which was prepared by the use of a home-made yttrium imprinted sorbent. The radiolabeling procedure was accomplished at 60 [Formula: see text] C during 12 h with a suitable radiochemical purity (95 ± 2% ITLC, 99 ± 0.5% HPLC) and specific activity of (1.0 ± 0.1 GBq/mmol). The obtained radio-labeled H2TPP in final formulation was kept for a week in order to investigate the complex stability. Accordingly, the partition coefficient was calculated as log [Formula: see text] 2.05. Furthermore, the biodistribution of the [Formula: see text]Y–TPP was determined in vital organs of normal wild-type rats using scarification studies. The kidneys could mostly remove the radio-complexes from the blood circulation and in less extends from the liver. As a result it is expected that due to its lipophilicity the higher mitochondrial and thus, tumor cell uptake of this radiolabeled porphyrin happens and therefore [Formula: see text]Y–TPP could act as an efficient potential agent for targeted therapy of tumor.