Reducing and Caping Agent Potential of Sun-Mediated Aqueous extract of Thymus Linearis for Green Synthesis of Silver Nanoparticles and their Effect on Phytopathogenic Bacteria

Abstract

In this study, silver nanoparticles were synthesized using sun-mediated aqueous extract of the stem, flower and leaf of the plant Thymus linearis. The effect of sunlight on extract absorbance is measured using UV–Vis spectroscopy. The resultant nanoparticle F-Ag–NPs, L-Ag–NPs, S-Ag–NPs were characterized using modern techniques including UV–Vis spectroscopy, ATR-FTIR, Zeta potential, Zeta size analyzer, XRD and SEM. The reaction takes place in presence of sunlight and the color changes from light yellow to red finally, dark brown was observed indicating the completion of the reaction. In the case of Flower extract, F-Ag–NPs produced maximum absorbance at a higher concentration of AgN[Formula: see text] with lambda max 403, and 405 nm at 5 mM and 10 mM respectively. In the case of stem and leaves extract, maximum absorbance was seen at a low concentration of AgN[Formula: see text] with lambda max range from 391 nm to 402 nm and 409–425 nm respectively. The ATR-FTIR spectrum confirms the presence of capping agents which are complemented by a negative zeta potential value. The Zeta sizer revealed the diameter (d-nm) of F-Ag–NPs, L-Ag–NPs, S-Ag–NPs, 73.26, 59.26 and 51.32 respectively. The Ag–NPs also showed anti-bacterial, DDPH, and ABTS activity.