Molecular Modification of AgNPs Synthetic Protein and Characterization of Synthetic AgNPs

Abstract

Nanosilver (AgNPs) exhibit a wide range of potential uses in the optical, antibacterial, and catalytic fields, and biologically produced AgNPs have a number of benefits. Our lab earlier identified the Lysinibacillus sphaericus protein NA33, which is involved in the synthesis of AgNPs. Nevertheless, NA33 produced AgNPs with poor dispersion, a high particle size, and low efficiency. In order to address these difficulties, we successfully produced SNA15, a highly effective AgNPs recombinant protein, was produced after molecular modification of NA33. SNA15 demonstrated good stability, with a yield of 87 mg SNA15 isolated from per liter of Escherichia coli fermentation broth. No apparent precipitation was observed after 7–5 days longer than the original protein—of storage in a 4[Formula: see text]C refrigerator. The generated AgNPs by SNA15, had particle sizes ranging from 3[Formula: see text]nm to 20[Formula: see text]nm, which were smaller than the original synthesized AgNPs of 30–50[Formula: see text]nm by NA33 and demonstrated spectrum antibacterial activity. There will be many opportunities for application in the medical and healthcare industries.