Urinary 8-hydroxydeoxyguanosine is a better biomarker of aging in non-smokers

Abstract

Objective: The aim of this study is finding an optimal and convenient aging marker. Method: The information of participants’ occupation, family background, life style, physical condition, etc. was obtained by a self-administered questionnaire. Spot urine and saliva samples were collected during the early morning after fasting overnight. 8-hydroxydeoxygunaosine (8-OHdG), 8-iso-prostaglandin F2[Formula: see text] (8-isoPGF2[Formula: see text]), advanced oxidation protein products (AOPPs), malondialdehyde (MDA), and dehydroepian drosterone sulfate (DHEA-S) were measured by ELISA. Results: Around 112 non-smokers (males/females [Formula: see text] 61/51; mean age [Formula: see text] 44.9 years) were eligible and analyzed in this study. The mean urinary 8-OHdG level was [Formula: see text] (ng/mmol, creatinine) and mean 8-isoPGF2[Formula: see text] level was [Formula: see text] (pg/mmoL, creatinine). In saliva, the mean level of AOPPs, MDA, and DHEA-S was [Formula: see text] ([Formula: see text]mol/L), [Formula: see text] ([Formula: see text]mol/L) and [Formula: see text] (ng/mL), respectively. After analysis in multiple regression model, urinary 8-OHdG was influenced by age and tea consumption ([Formula: see text]); urinary 8-isoPGF2[Formula: see text] level was correlated with gender ([Formula: see text]); salivary DHEA-S was related to gender ([Formula: see text]) and marital status ([Formula: see text]); AOPPs and MDA in saliva presented no relationships with those factors. Conclusion: Among healthy non-smokers, urinary 8-OHdG is the best aging biomarker comparing to other markers.