Optimization method to suppress background for imaging multiple planes

Abstract

3D live imaging is important for better understanding of biological processes. To obtain biological dynamic process, high imaging speed is required. In order to improve speed in 3D live imaging, simultaneous imaging of multiple planes throughout a 3D volume has been proposed. However, a main disadvantage of this method is the cross-talk from neighboring imaging planes. In this paper, we propose an optimization method to suppress background from neighboring imaging planes. A D-aperture is used to generate multiple light sheets. An optimization method to suppress background is presented. The simulation results demonstrated that the proposed method can be used to suppress the effectiveness of background from neighboring light sheets.