Affiliation:
1. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060, P. R. China
2. Laser Institute, Qilu University of Technology (Shandong Academy of Sciences), Qingdao Shandong 266000, P. R. China
Abstract
Multiphoton microscopy (MPM) is a powerful imaging technology for brain research. The imaging depth in MPM is partly determined by emission wavelength of fluorescent labels. It has been demonstrated that a longer emission wavelength is favorable for signal detection as imaging depth increases. However, there has been no comparison with near-infrared (NIR) emission. In order to quantitatively analyze the effect of emission wavelength on 3-photon imaging of mouse brains in vivo, we utilize the same excitation wavelength to excite a single fluorescent dye and simultaneously collect NIR and orange-red emission fluorescence at 828[Formula: see text]nm and 620[Formula: see text]nm, respectively. Both experimental and simulation results show that as the imaging depth increases, NIR emission decays less than orange-red fluorescent emission. These results show that it is preferable to shift the emission wavelength to NIR to enable more efficient signal collection deep in the brain.
Funder
National Natural Science Foundation of China
Science and Technology Innovation Commission of Shenzhen
Shenzhen Science and Technology Planning Project
Key R&D Program of Shandong Province
Major Innovation Projects for Integrating Science, Education & Industry of Qilu University of Technology
Natural Science Foundation of Shandong Province
Publisher
World Scientific Pub Co Pte Ltd
Subject
Biomedical Engineering,Atomic and Molecular Physics, and Optics,Medicine (miscellaneous),Electronic, Optical and Magnetic Materials
Cited by
4 articles.
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