Combination of multi-focus Raman spectroscopy and compressive sensing for parallel monitoring of single-cell dynamics

Author:

Li Zhenzhen1,Zhang Xiujuan23,Xiao Chengui4,Chen Da15ORCID,Huang Shushi2,Zhang Pengfei1ORCID,Wang Guiwen2ORCID

Affiliation:

1. School of Precision Instruments and Optoelectronics Engineering, Tianjin University, Tianjin 300072, China

2. Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China

3. College of Physics Science and Technology, Guangxi Normal University, 15 Yucai Road, Guilin, Guangxi 541004, China

4. Food Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen Academy of Inspection and Quarantine, Shenzhen, Guangdong 518045, China

5. Center for Aircraft Fire and Emergency, Civil Aviation University of China, Tianjin 300300, China

Abstract

To overcome the low efficiency of conventional confocal Raman spectroscopy, many efforts have been devoted to parallelizing the Raman excitation and acquisition, in which the scattering from multiple foci is projected onto different locations on a spectrometer’s CCD, along either its vertical, horizontal dimension, or even both. While the latter projection scheme relieves the limitation on the row numbers of the CCD, the spectra of multiple foci are recorded in one spectral channel, resulting in spectral overlapping. Here, we developed a method under a compressive sensing framework to demultiplex the superimposed spectra of multiple cells during their dynamic processes. Unlike the previous methods which ignore the information connection between the spectra of the cells recorded at different time, the proposed method utilizes a prior that a cell’s spectra acquired at different time have the same sparsity structure in their principal components. Rather than independently demultiplexing the mixed spectra at the individual time intervals, the method demultiplexes the whole spectral sequence acquired continuously during the dynamic process. By penalizing the sparsity combined from all time intervals, the collaborative optimization of the inversion problem gave more accurate recovery results. The performances of the method were substantiated by a 1D Raman tweezers array, which monitored the germination of multiple bacterial spores. The method can be extended to the monitoring of many living cells randomly scattering on a coverslip, and has a potential to improve the throughput by a few orders.

Funder

Key Technologies Research and Development Program

Publisher

World Scientific Pub Co Pte Ltd

Subject

Biomedical Engineering,Atomic and Molecular Physics, and Optics,Medicine (miscellaneous),Electronic, Optical and Magnetic Materials

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Recent advances in Raman spectroscopy for skin diagnosis;Journal of Innovative Optical Health Sciences;2023-03-04

2. Observing single cells in whole organs with optical imaging;Journal of Innovative Optical Health Sciences;2023-01

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