Optical manipulation of neuronal mitochondria using scanning optical tweezers
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Published:2023-06-30
Issue:
Volume:
Page:
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ISSN:1793-5458
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Container-title:Journal of Innovative Optical Health Sciences
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language:en
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Short-container-title:J. Innov. Opt. Health Sci.
Author:
Gong Zhiyong1ORCID,
Wu Tianli1,
Chen Xixi1,
Wang Ping2,
Guo Jinghui3,
Li Yuchao1,
Li Baojun1
Affiliation:
1. Guangdong Provincial Key Laboratory of Nanophotonic Manipulation, Institute of Nanophotonics, Jinan University, Guangzhou 511443, P. R. China
2. Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, P. R. China
3. School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, P. R. China
Abstract
Mitochondria play a crucial role in the physiological functions and energy metabolism of neurons, which can help in the understanding of complex biochemical reactions associated with various neurodegenerative diseases. Neurons, being highly differentiated terminal cells, require a greater number of mitochondria than ordinary cells to generate significant amounts of ATP, which is necessary for the growth of differentiated neuronal structures like axons and dendrites and the transmission of electrical signals along neuronal axons. Advancements in imaging technology, electrophysiology, and fluorescence targeting labeling have facilitated the study of mitochondrial movements in neurons and axons. However, disordered mitochondrial movements can hinder their analysis and characterization. Thus, it becomes necessary to artificially control their transport. Here, we demonstrate the utilization of scanning optical tweezers (SOTs) on the stable trapping and precise transport of soma or axon of neurons and enable. The presented method provides an optical approach to the control of mitochondria or other organelles in complex and variable biological environment.
Funder
the National Natural Science Foundation of China
National Key Research and Development Program of China
Guangdong Basic and Applied Basic Research Foundation
the Open Project Program of Wuhan National Laboratory for Optoelectronics
National Postdoctoral Program for Innovative Talents
China Postdoctoral Science Foundation
the Outstanding Innovative Talents Cultivation Funded Programs for Doctoral Students of Jinan University
Publisher
World Scientific Pub Co Pte Ltd
Subject
Biomedical Engineering,Atomic and Molecular Physics, and Optics,Medicine (miscellaneous),Electronic, Optical and Magnetic Materials
Cited by
2 articles.
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