Super-resolution fluorescence polarization microscopy

Author:

Zhanghao Karl12,Gao Juntao34,Jin Dayong5,Zhang Xuedian12,Xi Peng125

Affiliation:

1. Department of Biomedical Engineering, College of Engineering, Peking University, P. R. China

2. School of Optical-Electronic and Computer Engineering, Shanghai University of Science and Technology, P. R. China

3. Department of Automation, Tsinghua University, Beijing 100084, P. R. China

4. Bioinfomatics Division, TNLIST, MOE Key Laboratory of Bioinformatics and Center for Synthetic & System Biology, Tsinghua University, Beijing 100084, P. R. China

5. Faculty of Science, Institute for Biomedical Materials and Devices (IBMD) University of Technology, Australia

Abstract

Fluorescence polarization is related to the dipole orientation of chromophores, making fluorescence polarization microscopy possible to reveal structures and functions of tagged cellular organelles and biological macromolecules. Several recent super resolution techniques have been applied to fluorescence polarization microscopy, achieving dipole measurement at nanoscale. In this review, we summarize both diffraction limited and super resolution fluorescence polarization microscopy techniques, as well as their applications in biological imaging.

Publisher

World Scientific Pub Co Pte Lt

Subject

Biomedical Engineering,Atomic and Molecular Physics, and Optics,Medicine (miscellaneous),Electronic, Optical and Magnetic Materials

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