Synthesis, Characterization and Influence of Silver Nanoparticles Against Phases of Musca Domestica

Abstract

The achievement of this research was to study the influence of AgNPs on the life stages of Musca domestica. The supernatant of Trichoderma harzianum successfully reduces AgNO3 into AgNPs through the duration of incubation, as noticed by the color change from white to red. The absorbance of UV–visible light results in an absorbance peak at 418 nm for nanoparticle solutions of Ag particles. The scanning electron microscopy (SEM) image confirmed the formation of AgNPs with an average particle size of 46.63 nm without aggregation or adhesion. The microscopy of energy-dispersive X-ray (EDX) approved the formation of AgNPs with a high amount of some atoms of carbon and oxygen derivatives from components of fungus extract used to reduce AgNO3. The concentration of AgNPs prepared with serial dilutions of 5%, 10%, 15%, 20% and 25%, also with sterilized distilled water as a control and the influence of AgNPs on phases of [Formula: see text] domestica had been studied. The eggs of the studied insects were affected by 20% of AgNPs, which showed an increase in incubation time of 11 h without any hatched eggs at 25%. The larval stage of the insect was also influenced by AgNPs solution, which at 25% increased the duration of incubation to reach 11 h with 27 larval insects dead, compared with 7 h without any missing membrane in the larval stage of the insect. As well as the pupal phase of insects, we increased the period of incubation to 10 h with 7 h in control, and in addition, the number of pupal insects dead reached 23 in 25% concentration. Finally, the AgNPs also increased the number of adult insects to reach 27 at 25% concentration. The AgNPs approved the activity toward [Formula: see text] domestica and therefore considered it a preferable option for biological control.