SURFACE FABRICATION OF BIOTINYLATED POLYPEPTIDE MULTI-LAYER DESIGNED FOR SURFACE PLASMON RESONANCE SENSING-Reference-Cited by-同舟云学术

SURFACE FABRICATION OF BIOTINYLATED POLYPEPTIDE MULTI-LAYER DESIGNED FOR SURFACE PLASMON RESONANCE SENSING

Published:2004-12 Issue:03n04 Volume:13 Page:525-534
ISSN:0218-8635
Container-title:Journal of Nonlinear Optical Physics & Materials
language:en
Short-container-title:J. Nonlinear Optic. Phys. Mat.

Author:

JEONG JAE HYUN¹,KIM BYOUNG YUN¹,CHO SUNG HO¹,KIM JONG-DUK¹

Affiliation:

1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Guseong-dong, Yuseong-gu, Daejon 305-701, Republic of Korea

Abstract

The novel modification of gold surfaces with a biotinylated polypeptide multilayer formed by nonspecific electrostatic adsorption was designed for the SPR sensing platform. The multilayer of polypeptides (poly-L-lysine and poly(aspartic acid)) was prepared onto a negatively charged self-assembled monolayer of 11-mercaptoundecanoic acid (MUA) on gold film. The polypeptide multilayer was functionalized with biotin by directly grafting to poly(succinimide) (PSI). The specific binding of avidin to the biotinylated polypeptide multilayer (DS 2.7%) resulted in a shift in the refractive index of 0.003±9×10-4 with a surface density of 2.9 ng/mm2. This multilayer surface was found to provide a simple and effective method for the SPR sensing.

Publisher

World Scientific Pub Co Pte Lt

Subject

Physics and Astronomy (miscellaneous),Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials

Link

https://www.worldscientific.com/doi/pdf/10.1142/S0218863504002213

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