Affiliation:
1. Department of Bioengineering, University of Missouri, Columbia, MO 65211, USA
Abstract
Based on the insight that only living organisms can be killed (and that killing can proceed much faster than cell-growth), we present an approach for the detection of viable microorganisms that is much faster than currently used culture-based methods. We do so by using microchannel Electrical Impedance Spectroscopy (m-EIS) for real-time detection of cell-death on exposure to a killing-agent.m-EIS relies on the fact that when living-cells with non-zero membrane potentials are exposed to high-frequency AC-field, induced-charges accumulate at the membrane-interface. Cell-death is accompanied by a loss of membrane-potential, and hence charge-storage (capacitance).A proof-of-principle for a clinical-application (detection of living mycobacteria in sputum) is demonstrated. Mycobacterium smegmatis (doubling-time ∼3 hours) and Mycobacterium bovis BCG (doubling-time ∼20 hours) in artificial-sputum are both detected in [Formula: see text]3 hours when exposed to amikacin. Times-to-detection (TTDs) are ∼12 hours and ∼84 hours (3 1/2 days), respectively for culture-based detection using current technologies (BD-MGIT-960[Formula: see text]) for samples containing similar loads of M. smegmatis and M. bovis BCG
Funder
National Institutes of Health
National Science Foundation
Publisher
World Scientific Pub Co Pte Lt
Cited by
2 articles.
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