Microscopes and technologies for imaging cells and their protein networks: From nano to atomic scale resolution

Abstract

Advances in the high resolution microscopes and digital imaging technologies have allowed us to visualize diffusible signaling cascades from the protein receptors in the cell membrane to the nucleus. As multi-omics biology evolves over time, building predictable DNA-RNA-protein expression, protein-protein interaction networks and thereby spatiotemporal dynamics of molecular events will help us to uncover differences on cell morphology and physiology between normal and disease states. Here we present an update on optical, fluorescence and electron transmitted microscopes and mass spectrometer imaging technologies and various ways we can apply them for biological discovery and diagnose of human diseases.