NUMERICAL SIMULATION OF PRESSURE-INDUCED CELL PRINTING

Abstract

Nowadays, because of great biomedical applications of state-of-the art prototyping (bio-printing), many studies have been conducted in this field with focus on three-dimensional prototyping. There are several mechanisms for bio-printing of live cells such as piezoelectric and thermal and pneumatic inkjeting systems. Cell viability should be preserved during the bio-printing process. Lots of researches have been carried out to investigate and compare cell viability through different prototyping mechanisms. In order to quantify percentage of the cells that are killed during the proto-typing process, applied stresses on the cell and consequently its deformation should be calculated. A maximum strain energy density that the cell can tolerate is reported in the range of 25 Kj ⋅ m-3 to 100 Kj ⋅ m-3. This can be considered as a criteria to find the percentage of the damaged cells during the bio-printing processes. In this study, the bio-printing of the cell has been modeled and the cell viability have been investigated. Firstly, it is shown that in modeling of the bio-printing process, the effects of dynamic flow on calculating the applied stress on the cell is not negligible and must be considered. In the next step, the percentage of damaged endothelial cell aggregate under 80 kPa applied pressure (64 MPa/m) and 200 micron nozzle diameter is reported. Based on findings of this study, the percentage of endothelial cells viability under mentioned condition is reported 76%. The proposed method of this study can be utilized to examine the cell viability and performance of each prototyping systems.