A MODEL ON BACTERIAL RESISTANCE CONSIDERING A GENERALIZED LAW OF MASS ACTION FOR PLASMID REPLICATION

Abstract

Bacterial plasmids play a fundamental role in antibiotic resistance. However, a lack of knowledge about their biology is an obstacle in fully understanding the mechanisms and properties of plasmid-mediated resistance. This has motivated investigations of real systems in vitro to analyze the transfer and replication of plasmids. In this work, we address this issue with mathematical modeling. We formulate and perform a qualitative analysis of a nonlinear system of ordinary differential equations describing the competition dynamics between plasmids and sensitive and resistant bacteria. In addition, we estimated parameter values from empirical data. Our model predicts scenarios consistent with biological phenomena. The elimination or spread of infection depends on factors associated with bacterial reproduction and the transfer and replication of plasmids. From the estimated parameters, three bacterial growth experiments were analyzed in vitro. We determined the experiment with the highest bacterial growth rate and the highest rate of plasmid transfer. Moreover, numerical simulations were performed to predict bacterial growth.