THE ELEMENTAL ANALYSIS OF IgE-SENSITIZED RBL-2H3 CELLS USING IN-AIR MICRO-PIXE

Abstract

Rat basophilic leukaemia cells (RBL-2H3) were analyzed by in air micro-PIXE analysis to investigate the relationship between the distribution of elements and histamine release in immune system. RBL-2H3 cells were sensitized with monoclonal immunoglobuline E ( IgE ) and stimulated with antigen. In the stimulated cells, it is understood that the level of Ca 2+ increases in cytoplasm and then the degranulation or the release of chemical mediators occur. In this study, stimulated RBL-2H3 cells with antigen and control cells were analyzed. After the stimulation, the Na in the cells was increased. Moreover some stimulated cells showed the characteristic distribution of sulfur that is not accompany with the distribution of phosphorus. In the same measurement, the aggregations of Na , Ca , Fe , and Zn were observed.