Application of a thin metallic film evaporated onto a sample surface as an internal Standard for determination of trace elements in biological samples by PIXE

Abstract

The purpose of this study is to analyze quantitatively trace elements in human organs without decomposing samples. A palladium thin layer was deposited onto a sample on carbon adhesive tape using a metal ion sputtering equipment. The Pd film was used as an internal standard to determine concentrations of trace elements in human liver. Bovine Liver was applied as the reference standard. Five elements Cu , Zn , Mn , Se and Fe were measured by this method. The values of Copper, Zinc and iron concentrations determined by PIXE using this method agree well with those by ICP-AES. It can he asserted that this method is quite useful in the study of non-destructive PIXE analysis.