A subtle calculation method for nanoparticle’s molar extinction coefficient: The gift from discrete protein-nanoparticle system on agarose gel electrophoresis

Author:

Zhong Ruibo1,Yuan Ming1,Gao Haiyang1,Bai Zhijun1,Guo Jun1,Zhao Xinmin1,Zhang Feng1

Affiliation:

1. Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot 010018, P. R. China

Abstract

Discrete biomolecule-nanoparticle (NP) conjugates play paramount roles in nanofabrication, in which the key is to get the precise molar extinction coefficient of NPs. By making best use of the gift from a specific separation phenomenon of agarose gel electrophoresis (GE), amphiphilic polymer coated NP with exact number of bovine serum albumin (BSA) proteins can be extracted and further experimentally employed to precisely calculate the molar extinction coefficient of the NPs. This method could further benefit the evaluation and extraction of any other dual-component NP-containing bio-conjugates.

Funder

National Natural Science Foundation of China

National Natural Science Foundation of China (CN)

Inner Mongolia Grassland Talent

Natural Science Foundation of Inner Mongolia Autonomous Region of China

Inner Mongolia Agricultural University

Publisher

World Scientific Pub Co Pte Lt

Subject

General Materials Science

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