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5. Data were subjected to a paired t-test or one-way ANOVA, where means were separated by Tukey's Honestly Significant Difference procedure at the probability level of 0.10 using JMP software (JMP The Statistical Discovery Software, 2005, SAS Institute Inc., Cary, NC). Statistical differences are reported at a 90% probability level (or greater) to account for chamber and animal variations that will allow for the recognition of differences between treatments based on limitations encountered in these experiments. Analyses of percentages were performed following transformation using arcsine of their square root.