İnsan Pankreas Kanserinde Borik Asit Uygulaması ile Bazı IncRNA’ların ve İnvazyonun Baskılanması

Abstract

Objective: It was aimed to investigate the effects of boric acid on some lncRNAs and invasion in PANC-1 and MIA PaCa-2 pancreatic cancer cells. Materials and Methods: The effects of boric acid on cell viability and invasion were investigated using XTT test and invasion chambers, respectively. lncRNA H19 and UCA1 expressions were evaluated in pancreatic cancer using TCGA data. Its effects on expressions of these lncRNAs and invasion genes were determined by qRT-PCR analysis. Results: The IC50 doses of boric acid were calculated as 14.25 mM in PANC-1 cells and 15.71 mM in MIA PaCa-2. TCGA data showed that H19 and UCA1 expressions were elevated in pancreatic cancer. H19 and UCA1 lncRNA levels were decreased with boric acid treatment. In addition, boric acid increased CDH1 and TIMP1 in both cell lines. However, it suppressed CDH2 expression. Boric acid increased TIMP2 in PANC-1 cells and TIMP3 expression in MIA PaCa-2 cells. In the invasion test, boric acid significantly suppressed invasion in both cells. Conclusions: Boric acid suppressed H19 and UCA1 expressions, which were found to be high in pancreatic cancer. In addition, it showed an anti-invasive effect by changing the expressions of genes that are important in invasion.