Prostat Kanserinde Yağ Damlacık Oluşumu ile İlişkili PLA2G7, UCP2 ve NEDD4L Genlerinin Protein Ekspresyonlarının Araştırılması

Abstract

Objective: Prostate cancer (PCa) is characterised by dysregulated lipid metabolism. PCa cells store lipids as lipid droplets and use them to facilitate proliferation and growth. In this study, we aim to investigate the expression levels of PLA2G7, UCP2 and NEDD4L proteins, which are involved in lipid droplet metabolism, in PC3 (advanced metastatic) and DU145 (intermediate metastatic) PCa cells. Materials and Methods: DU145 and PC3 cells were cultured in a high-glucose DMEM medium containing 10% FBS, 1% penicillin-streptomycin, and 1% non-essential amino acid. The expression levels of PLA2G7, UCP2, and NEDD4L protein were assessed with ELISA assay. Results: There was no significant difference in the protein level of PLA2G7 between the PC3 and DU145 cells (p>0.05), while the protein level of UCP2 increased in the PC3 cell line significantly (p<0.05). The protein level of NEDD4L decreased significantly in the DU145 cell line when compared to the PC3 cell line (p<0.05). Conclusions: As a result of this study, the UCP2 gene might play a role in the progression of prostate cancer, and there could be a relationship between NEDD4L and cell proliferation control.