Prevalence of Closely Related Candida albicans Species among Patients with Vulvovaginal Candidiasis in Southern Poland Based on the hwp1 Gene Amplification-Reference-Cited by-同舟云学术

Prevalence of Closely Related Candida albicans Species among Patients with Vulvovaginal Candidiasis in Southern Poland Based on the hwp1 Gene Amplification

Published:2023-03-01 Issue:1 Volume:72 Page:69-77
ISSN:2544-4646
Container-title:Polish Journal of Microbiology
language:en
Short-container-title:

Author:

KLESIEWICZ KAROLINA¹^ORCID,MROWIEC PAULINA¹^ORCID,KANIA KATARZYNA¹^ORCID,SKIBA-KUREK IWONA¹^ORCID,BIAŁECKA JOANNA¹²,NAMYSŁ MAGDALENA³,MAŁEK MARIANNA¹^ORCID

Affiliation:

1. Department of Pharmaceutical Microbiology , Jagiellonian University Medical College , Cracow , Poland

2. Centre of Microbiological Research and Autovaccines , Cracow , Poland

3. University Hospital , Cracow , Poland

Abstract

Abstract Candida albicans remains the most common species isolated from women with vulvovaginal candidiasis. However, closely related species such as Candida africana and Candida dubliniensis may also occur, although they are often misidentified. The aim of the study was to confirm the phenotypic identification of C. albicans and its closely related species isolated from women with genital tract infections by amplification of the hwp1 (hyphal wall protein 1) gene in a PCR assay. We report a detailed molecular identification of C. albicans and its closely related species among 326 patients in the Małopolska region, Poland. Initial phenotypic identifications were confirmed by amplification of the hwp1 gene. Based on molecular analysis, we revealed 307 strains (94.17%) as C. albicans and 17 as C. dubliniensis (5.22%). No strain of C. africana was detected. Two patients h ad co-infection with C. albicans and C. dubliniensis (0.61%). A PCR assay targeting the hwp1 gene was reliable for correctly identifying species among the C. albicans complex.

Publisher

Polish Society of Microbiologists

Subject

Microbiology (medical),Applied Microbiology and Biotechnology,General Medicine,Microbiology

Link

https://www.sciendo.com/pdf/10.33073/pjm-2023-011

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