Affiliation:
1. Institute of Seafood , Zhejiang Gongshang University , Hangzhou , China
2. The Joint Key Laboratory of Aquatic Products Processing of Zhejiang Province , Hangzhou , China
3. Huzhou Customs , Huzhou , China
Abstract
Abstract
The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) among the aquatic products collected in Zhejiang, China. A total of 136 presumptive ARB picked up from six aquatic samples were classified into 22 genera and 49 species based on the 16S rDNA sequencing. Aeromonas spp., Shewanella spp., Acinetobacter spp., Myroides spp., Pseudomonas spp., and Citrobacter spp. accounted for 80% of the ARB. Among them, 109 isolates (80.15%) exhibited resistance to at least one antibiotic. Most isolates showed resistance to not only the originally selected drug but also to one to three other tested drugs. The diversity of ARB distributed in different aquatic products was significant. Furthermore, the resistance data obtained from genotypic tests were not entirely consistent with the results of the phenotypic evaluation. The genes qnrS, tetA, floR, and cmlA were frequently detected in their corresponding phenotypic resistant isolates. In contrast, the genes sul2, aac(6’)-Ib, and bla
PSE were less frequently found in the corresponding phenotypically resistant strains. The high diversity and detection rate of ARB and ARGs in aquaculture might be a significant threat to the food chains closely related to human health.
Publisher
Polish Society of Microbiologists
Subject
Microbiology (medical),Applied Microbiology and Biotechnology,General Medicine,Microbiology
Reference34 articles.
1. Bureau of Fisheries. [2020 National Fishery Economic Statistical Bulletin] (in Chinese) [Internet]. Beijing (China); Ministry of Agriculture and Rural Affairs: 2021 [cited 2022 Oct 26]. Available from http://www.moa.gov.cn/xw/bmdt/202107/t20210728_6372958.htm
2. Cao H, Chen S, Lu L, An J. Shewanella algae: an emerging pathogen of black spot disease in freshwater-cultured whiteleg shrimp (Penaeus vannamei). Isr J Aquac Bamidgeh. 2018 Jan;(70). https://doi.org/10.46989/001c.20952
3. Chenia HY. Prevalence and characterization of plasmid-mediated quinolone resistance genes in Aeromonas spp. isolated from South African freshwater fish. Int J Food Microbiol. 2016 Aug;231:26–32. https://doi.org/10.1016/j.ijfoodmicro.2016.04.030
4. CLSI. Performance standards for antimicrobial susceptibility testing. 28th ed. CLSI supplement M100. Wayne (USA): Clinical and Laboratory Standards Institute; 2018.
5. Cui G, Liu Z, Xu W, Gao Y, Yang S, Grossart HP, Li M, Luo Z. Metagenomic exploration of antibiotic resistance genes and their hosts in aquaculture waters of the semi-closed Dongshan Bay (China). Sci Total Environ. 2022 Sep;838(Pt 1):155784. https://doi.org/10.1016/j.scitotenv.2022.155784
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献