A Recombinase-Aided Amplification Assay for the Detection of <i>Chlamydia felis</i>-Reference-Cited by-同舟云学术

A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis

Published:2023-09-01 Issue:3 Volume:72 Page:339-343
ISSN:2544-4646
Container-title:Polish Journal of Microbiology
language:en
Short-container-title:

Author:

Liu Jian¹,Qian Weidong²,Wang Jian¹,Bai Yilan¹,Gui Yaping¹,Xia Luming¹,Gong Guohua¹,Ge Feifei¹,Shen Haixiao¹,Chang Xiaojing¹,Zhao Hongjin¹

Affiliation:

1. Shanghai Animal Disease Prevention and Control Center , Shanghai , China

2. School of Food and Biological Engineering , Shaanxi University of Science and Technology , Xi’an , China

Abstract

Abstract Chlamydia felis is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting C. felis. RAA can be performed in a closed tube at 39°C within 30 min. The detection limit was 10.6 copies of the C. felis plasmid DNA per reaction. No positive signals for other pathogens were detected. The coincidence rate of RAA and conventional PCR was 95.24% (20/21) and 100% (96/96) for positive and negative samples, respectively. The established RAA assay is a simple, rapid, highly sensitive, and specific method for detecting C. felis.

Publisher

Polish Society of Microbiologists

Subject

Microbiology (medical),Applied Microbiology and Biotechnology,General Medicine,Microbiology

Link

https://www.sciendo.com/pdf/10.33073/pjm-2023-029

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