Comparison of mCP and TSC Media to Enumerate Clostridium perfringens in Surface Water Samples

Author:

Matuszewska Renata1ORCID,Mąka Łukasz1ORCID

Affiliation:

1. Department of Environmental Health and Safety National Institute of Public Health National Institute of Hygiene – National Research Institute , Warsaw , Poland

Abstract

Abstract The Clostridium perfringens bacteria are used to assess water quality as an indicator parameter. If detected, it can confirm the occurrence of past fecal contamination. Tests determining C. perfringens in water samples are usually performed by membrane filtration where filters are incubated on selective media under anaerobic conditions. Available media include mCP and TSC. The aim of this study was to compare the relative recovery of C. perfringens (including spores) from surface water samples and to determine the performance characteristics of the membrane filtration method using both media. The results showed that, although the procedure using the mCP medium was more sensitive and specific, higher recoveries were obtained in the tests based on the TSC medium.

Publisher

Polish Society of Microbiologists

Subject

Microbiology (medical),Applied Microbiology and Biotechnology,General Medicine,Microbiology

Reference28 articles.

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