Author:
ZHAI QINGZHI,ZHANG WEIYI,ZHANG ZHE,FU YURONG,LI YANG,WANG XUEQI,LI LI’AN,MENG YUANGUANG
Abstract
Persistent infection with high-risk human papillomavirus (HR-HPV)is the most important determinate in the development of cervical cancer, and cervical microecology can modulate cervical viral infec¬tion. However, few studies have been conducted on the microeco¬logical analysis of cervical diseases using strict physiological factors. This study investigated the characteristics and dynamics of cervical microecology in childbearing-age Chinese women with different degrees of HR-HPV-positive cervical lesions. A total of 168 subjects were selected according to the selection criteria, including healthy HPV-negative individuals (n = 29), HR-HPV-infected individuals (n = 29), low-grade squamous intraepithelial lesion individuals (LSIL, n = 32), high-grade squamous intraepithelial lesion indi¬viduals (HSIL, n = 40), and cervical cancer individuals (n = 38). We sampled cervical secretions from each subject and performed com¬parative analysis using the 16S rRNA sequencing method. Com¬parison analysis showed that Lactobacillus and Ignatzschineria were the dominant genera in the healthy group, while Gardnerella and Prevotella were more enriched in the disease groups. Based on the taxa composition, we roughly divided the development of cervical cancer into two phases: phase I was from healthy status to HR-HPV infection and LSIL; phase II was from LSIL to HSIL and cervical cancer. Different interactions among different genera were observed in different groups. Prevotella inhibited the abundance of Lactoba¬cillus in the healthy group, while Prevotella inhabited the abundance of Gardnerella in the other groups. In the HR-HPV infection group, Ignatzschineria and Enterococcus showed a positive interaction but dissociated with the increase in cervical lesions, which might even¬tually lead to a continuous decrease in the abundances of Lactoba¬cillus and Ignatzschineria.
Publisher
Polish Society of Microbiologists
Subject
Microbiology (medical),Applied Microbiology and Biotechnology,General Medicine,Microbiology
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献