Identification and Antibiotic Susceptibility of Eggerthella lenta in Bloodstream Infections

Author:

Li Xiangyun1,Xu Enjun1,Shi Cuixiao1,Qiao Guanhua2,Chen Shuyi2,Xu Yuanhong1,Liu Yajing345,Bao Xundi6

Affiliation:

1. Department of Laboratory Medicine, the First Affiliated Hospital of Anhui Medical University , Hefei , People’s Republic of China

2. Department of Laboratory Medicine, Anhui Medical University , Hefei , People’s Republic of China

3. Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University , Hefei , People’s Republic of China

4. NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Anhui Medical University , Hefei , People’s Republic of China

5. Anhui Province Key Laboratory of Reproductive Health and Genetics, Anhui Medical University , Hefei , People’s Republic of China

6. Department of Laboratory Medicine, Anhui Chest Hospital , Hefei , People’s Republic of China

Abstract

Abstract The identification and antibiotic susceptibility of two clinical isolates of Eggerthella lenta from bloodstream infections were determined. This microorganism is rarely pathogenic, and the findings are presented here to promote the detection and awareness of this infection. The bacteria were obtained from one patient with pressure sores and another with a malignant gastric tumor. Smears were prepared, stained, and examined by microscopy. Single colonies were analyzed by Gram staining, MALDI-TOF MS, and the 16S rRNA gene sequencing. Antibiotic sensitivity was assessed by the agar dilution method. The bacilli were found to be Gram-positive, and the MS results showed 99.8% homology with E. lenta. It was confirmed by gene sequencing. Antibiotic susceptibility tests demonstrated that E. lenta was sensitive to piperacillin-tazobactam, ampicillin-sulbactam, imipenem, meropenem, metronidazole, clindamycin, and vancomycin. This study could increase awareness of this rare infection.

Publisher

Polish Society of Microbiologists

Subject

Microbiology (medical),Applied Microbiology and Biotechnology,General Medicine,Microbiology

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