Molecular Genetic Analysis of the Complete Genome of Tick-Borne Encephalitis Virus (Siberia Subtype): Modern Kolarovo-2008 Isolate-Reference-Cited by-同舟云学术

Molecular Genetic Analysis of the Complete Genome of Tick-Borne Encephalitis Virus (Siberia Subtype): Modern Kolarovo-2008 Isolate

Published:2011-08-20 Issue:4(110) Volume: Page:44-48
ISSN:0370-1069
Container-title:Problems of Particularly Dangerous Infections
language:
Short-container-title:Problemy Osobo Opasnykh Infektsii

Author:

Chausov E. V.¹,Ternovoy V. A.¹,Protopopova E. V.¹,Konovalova S. N.¹,Kononova Yu. V.¹,Tupota N. L.¹,Moskvitina N. S.²,Romanenko V. N.²,Ivanova N. V.²,Bol'shakova N. P.²,Leonova G. N.³,Loktev V. B.¹

Affiliation:

1. State Research Centre of Virology and Biotechnology Vector

2. Tomsk State University

3. RAMS Siberian Branch Research Institute of Epidemiology and Microbiology

Abstract

Determined is the complete genome sequence of Kolarovo-2008 strain (Siberia subtype) of Tick-borne encephalitis virus (TBEV), isolated from a tick in the suburbs of the Tomsk city. Nucleotide sequence analysis testifies of the fact that the level of genetic differences within the Siberian subtype of TBEV amounts to 10 % of the nucleotide sequence and to 7 % of amino-acid sequence for certain virus genes. 3'-HTO of the genome of Siberian subtype has the highest rate of variability and the homology level ranging from 65 to 97 %. Kolarovo-2008 and Vasilchenko (isolated in Novosibirsk in 1969) strains have the highest level of genome homology. The level of dissimilarity between the two Tomsk strains is substantially higher: the total number of amino-acid substitutions in Tomsk Zausaev and Kolarovo-2008 strains equals to 124, and 3'HTO level of homology is 79 %. Identified genetic variability of the Siberian subtype of TBEV is of a great importance for further development and enhancement of tick-borne encephalitis virus diagnostics.

Publisher

Russian Research Anti-Plague Institute Microbe

Subject

Infectious Diseases,Microbiology (medical),Immunology,Microbiology,Epidemiology

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