Affiliation:
1. Irkutsk Research Anti-Plague Institute of Siberia and Far East
2. National Centre of Zoonotic Infections
Abstract
Advanced molecular-genetic methods for the diagnosis and typing of Yersinia pestis ssp. pestis in the field and clinical material are used for epidemiological surveillance of plague in the Saylyugem natural focus. The aim of the work was to study the spatial genotypic structure of Y. pestis ssp. pestis in the transboundary Saylyugem natural plague focus using MLVA25 typing. Materials and methods. The MLVA25 typing of 160 strains of Y. pestis ssp. Pestis isolated in the Saylyugem natural plague focus in 2012–2021 was carried out. Phylogenetic tree construction was performed with the help of UPGMA and MST methods. Results and discussion. The Y. pestis ssp. pestis strains isolated from the Saylyugem natural plague focus were differentiated into 15 MLVA types by the 25 VNTR loci cluster analysis. The studied strains form a homogeneous complex of MLVA25 types without marked geographical distribution across seven spatial groups. The analysis of the frequency of occurrence of the tandem repeats number for three variable loci of Y. pestis ssp. pestis strains shows the significant differences between the samples from the Mongolian and Russian parts of the Saylyugem natural plague focus. The most pronounced differences in spatial genotypic structure are traced through the yp4280ms62 locus.
Publisher
Russian Research Anti-Plague Institute Microbe
Subject
Infectious Diseases,Microbiology (medical),Immunology,Microbiology,Epidemiology
Reference16 articles.
1. Zhao S.S., Pulati Y., Yin X.P., Li W., Wang B.J., Yang K., Chen C.F., Wang Y.Z. Wildlife plague surveillance near the China-Kazakhstan border: 2012–2015. Transbound. Emerg. Dis. 2017; 64(6):e48–e51. DOI: 10.1111/tbed.12603.
2. Wang P., Shi L., Zhang F., Guo Y., Zhang Z., Tan H., Cui Z., Ding Y., Liang Y., Liang Y., Yu D., Xu J., Li W., Song Z. Ten years of surveillance of the Yulong plague focus in China and the molecular typing and source tracing of the isolates. PLoS Negl. Trop. Dis. 2018; 12(3):e0006352. DOI: 10.1371/journal.pntd.0006352.
3. Verzhutsky D.B. [Activation of natural plague foci in Central Asia: groundless fears or a real threat]. Priroda Vnutrenney Azii. [Nature of Inner Asia]. 2018; 1:7–18. DOI: 10.18101/2542-0623-2018-1-7-18.
4. Korzun V.M., Chipanin E.V., Denisov A.V., Kurepina N.Yu. [Areal and spatial structure of the population of the Mongolian pika (Ochotona pallasi, Ochotonidae, Lagomorpha) in the South-Eastern Altai]. Baikal’sky Zoologichesky Zhurnal [Baikal Zoological Journal]. 2017; (1):72–82.
5. Popova A.Yu., Kutyrev V.V., editors. [Ensuring Epidemiological Welfare in Natural Plague Foci in the Territory of CIS Countries and Mongolia under Current Conditions]. Izhevsk: LLC “Print”; 2018. 336 p.