Studying the Effect of Experimental Bases on the Growth Quality of Liquid Nutritional Media for Submerged Cultivation of Plague Microbe Vaccine Strain-Reference-Cited by-同舟云学术

Studying the Effect of Experimental Bases on the Growth Quality of Liquid Nutritional Media for Submerged Cultivation of Plague Microbe Vaccine Strain

Published:2022-04-19 Issue:1 Volume: Page:71-76
ISSN:2658-719X
Container-title:Problems of Particularly Dangerous Infections
language:
Short-container-title:Problemy Osobo Opasnykh Infektsii

Author:

Abzaeva N. V.¹^ORCID,Gostishcheva S. E.¹^ORCID,Startseva O. L.¹^ORCID,Katunina L. S.¹^ORCID,Kovalev D. A.¹^ORCID,Ivanova G. F.¹^ORCID,Kostrominov A. V.¹^ORCID,Kurilova A. A.¹^ORCID

Affiliation:

1. Stavropol Research Anti-Plague Institute

Abstract

The aim of the study was to investigate the effect of experimental bases on the growth qualities of liquid nutrient media at the stage of obtaining the biomass of plague microbe vaccine strain using submerged cultivation.Materials and methods. Yersinia pestis EV NIIEG vaccine strain was used in the work. The cultivation was carried out in a 5 L bioreactor with automatic stirrer control. We used 28 variants of nutrient media obtained through combining five types of bases and six growth stimulants. Nutrient media without the addition of growth-stimulating additives were used as controls. The following parameters were assessed in the yield biomass: the total number of microbial cells, pH, the percentage of viable microbial cells.Results and discussion. On experimental nutrient media, biomasses of the plague microbe vaccine strain have been produced using submerged cultivation. After evaluating bacterial suspensions by the main indicators, the quality of the obtained suspensions has been compared depending on the nutrient medium used. The most promising bases identified are pancreatic hydrolyzate of casein with dry enzymatic peptone and acid hydrolyzate of corn syrup, especially in combination with such growth stimulants as sodium sulfite, ferrous ammonium sulfate or ammonium molybdate.

Publisher

Russian Research Anti-Plague Institute Microbe

Subject

Infectious Diseases,Microbiology (medical),Immunology,Microbiology,Epidemiology

Reference22 articles.

1. Eremin S.A., Volokh O.A., Shepelev I.A., Dalvadyants S.M., Dyatlov I.A. [Development of new technological schemes and scaling up the processes for obtaining antigens of plague and tularemia microbes]. Problemy Osobo Opasnykh Infektsii [Problems of Particularly Dangerous Infections]. 2006; (92):58–61.

2. Sharov D.A., Leshchenko A.A., Bagin S.V., Mokhov D.A., Logvinov S.V., Krupin V.V., Ezhov A.V., Lazykin A.G., Biryukov V.V. [Optimization of concentrating microbial cells in the techno logy of plague vaccines]. Biopreparaty. Profilaktika, Diagnostika, Lechenie [BioPreparations. Prevention, Diagnosis, treatment]. 2019; 19(1):50–5. DOI: 10.30895/2221-996X-2019-19-1-50-55.

3. Zaitsev V.V., Dremach G.E., Zaitseva A.V., Safronenko L.V., Tunkel’ V.S. [Experimental data on the cultivation of some bacteria in the gas-vortex bioreactor “Biok”]. Kommercheskaya Biotekhnologiya [Commercial Biotechnology]. [Internet]. Available from: http://www.cbio.ru/page/43/id/3500/.

4. Sharov D.A., Leshchenko A.A., Bagin S.V., Logvinov S.V., Ezhov A.V., Lazykin A.G., Mokhov D.A., Krupin V.V., Ziganshin A.R. [Improving the technology of production of live plague vaccine]. Vestnik Voisk RKhB Zashchity [Bulletin of the RCB Protection Troops]. 2017; 1(3):30–7.

5. Kaminsky D.I., Lobanov V.V., Rozhkov K.K., Mazrukho A.B. [Improvement of nutrient media for growing some pathogens of dangerous infectious diseases]. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii [Journal of Microbiology, Epidemiology and Immunobiology]. 2017; (2):104–10. DOI: 10.36233/0372-9311-2017-2-104-110.

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Possibility of Using Disposable Polymeric Containers in the Production Technology of Live Plague Vaccine;Problems of Particularly Dangerous Infections;2024-01-10