Multilocus Sequence-Typing of Anthrax Microbe Strains Isolated in Russia and Neighboring Countries
-
Published:2021-04-16
Issue:1
Volume:
Page:95-102
-
ISSN:2658-719X
-
Container-title:Problems of Particularly Dangerous Infections
-
language:
-
Short-container-title:Problemy Osobo Opasnykh Infektsii
Author:
Goncharova Yu. O.1ORCID, Bakhteeva I. V.1ORCID, Mironova R. I.1ORCID, Bogun A. G.1, Khlopova K. V.1ORCID, Timofeev V. S.1ORCID
Affiliation:
1. State Research Center of Applied Microbiology and Biotechnology
Abstract
Objective – genotyping by multilocus sequence-typing (MLST) and phylogenetic analysis of 40 Bacillus anthracis strains isolated in Russia and neighboring countries.Materials and methods. In this study, the sequences of seven housekeeping genes of B. anthracis strains were assembled based on the data of a whole genome new generation sequencing, after which the identified mutations and their coordinates were described. The obtained sequences were used for genotyping of the investigated sample using the MLST method. The results are compared with the data presented in PubMLST database. A phylogenetic analysis was performed for the in silico fused sequences of the seven loci of the identified sequence types. The MEGA 7.0 software package was used to build the dendrograms.Results and discussion. Two sequence types (ST) have been found in the examined sample: 35 strains belong to ST-1, and five strains that differed by one common mutation at the glpF locus – to ST-3 (according to PubMLST coding), which emphasizes the genetic separation of this group of strains. One strain has a unique mutation in the gmk gene located outside the region used for MLST.
Publisher
Russian Research Anti-Plague Institute Microbe
Subject
Infectious Diseases,Microbiology (medical),Immunology,Microbiology,Epidemiology
Reference25 articles.
1. Carroll L.M., Kovac J., Miller R.A., Wiedmann M. Rapid, high-throughput identification of anthrax-causing and emetic Bacillus cereus group genome assemblies via BTyper, a computational tool for virulence-based classification of Bacillus cereus group isolates by using nucleotide sequencing data. Appl. Environ. Microbiol. 2017; 83(17):e01096-17. DOI: 10.1128/AEM.01096-17. 2. Marinin L.I., Dyatlov I.A., Shishkova N.A., Gerasimov V.N. [Anthrax Cattle Burial Grounds: Problems and Solutions]. Moscow: “Dynasty”; 2017. 215 p. 3. Hoffmann C., Zimmermann F., Biek R., Kuehl H., Nowak K., Mundry R., Agbor A., Angedakin S., Arandjelovic M., Blankenburg A., Brazolla G., Corogenes K., Couacy-Hymann E., Deschner T., Dieguez P., Dierks K., Düx A., Dupke S., Eshuis H., Formenty P., Yuh Y.G., Goedmakers A., Gogarten J.F., Granjon A.C., McGraw S., Grunow R., Hart J., Jones S., Junker J., Kiang J., Langergraber K., Lapuente J., Lee K., Leendertz S.A., Léguillon F., Leinert V., Löhrich T., Marrocoli S., Mätz-Rensing K., Meier A., Merkel K., Metzger S., Murai M., Niedorf S., De Nys H., Sachse A., van Schijndel J., Thiesen U., Ton E., Wu D., Wieler L.H., Boesch C., Klee S.R., Wittig R.M., Calvignac-Spencer S., Leendertz F.H. Persistent anthrax as a major driver of wildlife mortality in a tropical rainforest. Nature. 2017; 548(7665):82–6. DOI: 10.1038/nature23309. 4. Helgason E., Tourasse N.J., Meisal R., Caugant D.A., Kolsto A.B. Multilocus sequence typing scheme for bacteria of the Bacillus cereus group. Appl. Environ. Microbiol. 2004; 70(1):191–201. DOI: 10.1128/aem.70.1.191-201.2004. 5. Agren J., Hamidjaja R.A., Hansen T., Ruuls R., Thierry S., Vigre H., Janse I., Sundstrom A., Segerman B., Koene M., Löfström C., Van Rotterdam B., Derzelle S. In silico and in vitro evaluation of PCR-based assays for the detection of Bacillus anthracis chromosomal signature sequences. Virulence. 2013; 4(8):671–85. DOI: 10.4161/viru.26288.
|
|