Detection of Phage-Induced Mobile Genetic Elements in Strains of <i>Vibrio cholerae</i> O1 Biovar El Tor

Author:

Zadnova S. P.1ORCID,Plekhanov N. A.1ORCID,Spirina A. Yu.1ORCID,Shvidenko I. G.1,Savel’ev V. N.2ORCID

Affiliation:

1. Russian Research Anti-Plague Institute “Microbe”

2. Stavropol Research Anti-Plague Institute

Abstract

The acquisition of new mobile genetic elements contributes to the genetic diversity of Vibrio cholerae strains. An important role in this process belongs to the genetic material obtained from phages. The aim of this work was to identify phage-induced PLE islands in strains of V. cholerae O1 serogroup and to determine the resistance of isolates with and without those mobile genetic elements to the lytic activity of the diagnostic cholera El Tor bacteriophage. Materials and methods. Whole genomes nucleotide sequences of toxigenic and non-toxigenic V. cholerae O1 strains presented in the NCBI GenBank were used for the work. Bioinformatic analysis was performed using the BLAST algorithm, MEGA X (or BioEdit v. 7.0.9.0). The test with phages was carried out according A. Gratia technique. Results and discussion. The analysis of 39 toxigenic strains imported to the territory of the Russian Federation and neighboring countries has revealed one strain of V. cholerae O1 of the classical biovar containing the PLE5 island, and 13 strains of V. cholerae O1 of the El Tor biovar containing the PLE4 island. PLE islands have not been found in non-toxigenic strains. It is shown that strains with PLE4 belong to V. cholerae O1 genovariants of the El Tor biovar and have the ctxB1 gene allele. Isolates with this mobile element caused sporadic cases of the disease in 1994–1999, as well as cholera outbreaks in the Russian Federation (in 1993–1994, in 1998 – Dagestan, and 1993 – Tatarstan) and Ukraine (1994–1995). It has been suggested that, perhaps, the presence of the PLE4 island makes a certain contribution to the resistance of V. cholerae O1 strains of the El Tor biovar to the diagnostic cholera El Tor phage (55.6 % of phage-resistant isolates were detected), but there are other mechanisms that have not yet been identified. Thus, the data on the presence of new mobile genetic elements in the genome of earlier imported toxigenic strains of V. cholerae O1, biovar El Tor have been obtained, which expands information about their genetic organization.

Publisher

Russian Research Anti-Plague Institute Microbe

Subject

Infectious Diseases,Microbiology (medical),Immunology,Microbiology,Epidemiology

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Assessment of the Effectiveness of Cholera Bacteriophages for Prevention of Experimental Cholera;Problems of Particularly Dangerous Infections;2024-07-03

2. Systems of Phage Resistance in <I>Vibrio cholerae</I> Strains;Problems of Particularly Dangerous Infections;2024-06-24

3. Analysis of Antiphage Systems in Vibrio Cholerae O1 El Tor Biotype Strains;ЗДОРОВЬЕ НАСЕЛЕНИЯ И СРЕДА ОБИТАНИЯ - ЗНиСО / PUBLIC HEALTH AND LIFE ENVIRONMENT;2023-11

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