Affiliation:
1. CUMHURİYET ÜNİVERSİTESİ, FEN FAKÜLTESİ, MOLEKÜLER BİYOLOJİ VE GENETİK BÖLÜMÜ, MOLEKÜLER BİYOLOJİ VE GENETİK ANABİLİM DALI
Abstract
Neodiprion is a genus belonging to the small sawfly family Diprionidae, feeding the plant family Pinaceae entirely. Here, the complete mitogenome of the redheaded pine sawfly Neodiprion lecontei (Hymenoptera: Diprionidae) was assembled, annotated as third party annotation from the raw genome dataset of N. lecontei and comparatively characterised. The length of N. lecontei mitogenome was 16,067 bp in size, with an AT content of 81.32%. The initiation codons of protein coding genes (PCGs) are ATN (except for nad6 (TTA-Phe), while termination codons are TAA or T−. tRNA genes favoured usual anticodons except for trnS1 which preferred an unusual anticodon GCU. Compared with the Neodiprion sertifer mitogenome, the ARNS1EF gene cluster was rearranged as RAS1RNEF and trnR gene has a duplicated copy, revealing a new event not formerly reported in Symphyta. The phylogeny confirms the position of N. lecontei within the family of Diprionidae and supports the monophyly of included genera and families in Tenthredinoidea.