A SIMPLE AND SELECTIVE METHOD FOR SIMULTANEOUS DETERMINATION OF PATULIN AND 5- HMF IN HONEY AND APPLE JUICE BY HPLC-UV

Abstract

A selective and sensitive method using a high-performance liquid chromatography-UV detector (HPLC-UV) for the simultaneous determination of Patulin (PAT) and 5-(hydroxymethyl)furfural (5-HMF) in honey and apple juice was developed. The sample preparation technique QuEChERS was applied to increase the sensitivity and selectivity. The linear ranges for PAT and 5-HMF detected by the method were 2.0 ppb ÷ 200.0 ppb and 2.0 ppm ÷ 20 ppm. The results of method validation show that the recovery efficiency for PAT ranged from 92.8% ÷ 98.2% with % RSDr from 0.56% ÷ 0.63% and from 97.9% ÷ 101.2% with %RSD 0.39% ÷ 0.67% for 5-HMF and LOD and LOQ values of the method for PAT and 5-HMF detection were respectively: 0.6 and 2.0 ppb; 0.6 and 2.0 ppm. The influence of the matrix effect on the accuracy, repeatability, and recovery of the method was insignificant. The proposed method was applied to quantify the content of PAT, and 5-HMF in actual samples, collected in Ho Chi Minh City, Vietnam. The result showed that PAT was not found in honey samples but was detected in 5 of 10 apple juice samples. In addition, 5-HMF was detected in 3 of 10 honey samples and 10 of 10 apple juice samples.