Urban ecology of arboviral mosquito vectors along the Kenyan coast

Abstract

ABSTRACTBackgroundThe emergence and re-emergence of arboviral infections particularly Chikungunya, dengue hemorrhagic fever, rift valley fever, and yellow fever in humans around the world threatens global health and security. The purpose of this study was to determine the urban ecology of the common arboviral mosquito vectors in urban Coastal Kenya areas.Materials and MethodsThe current study was conducted in urban settings of Kilifi and Mombasa counties in coastal Kenya in 2016 to 2017. Adult mosquitoes were collected both indoors and outdoors by CDC light traps and BG-Sentinel traps respectively. All blood fed mosquitoes were tested for blood meal sources by an Enzyme Linked Immunosorbent Assay (ELISA). Mosquito larvae were collected using standard dippers and pipettes. Egg survivorship in dry soil was evaluated by collecting of soil samples from dry potential breeding habitats, watering them for hatching and rearing of the eventual larvae to adults. Mosquitoes were screened forFlavivirus, Alphavirus,andPhlebovirusarboviruses using Reverse Transcriptase quantitative Polymerase Chain Reaction (RT qPCR).ResultsA total of 3,264 adult mosquitoes belonging to ten species ofCulex, AedesandAnopheleswere collected. Overall, the predominant species wereCx. quinquefasciatus72.4% (n=2,364) andAe. aegypti,25.7%, (n=838). A total of 415 breeding habitat types were identified indoors (n=317) and outdoors (n=98). The most productive habitat types in both indoors and outdoors were: assorted small containers, water tanks, drainages, drums and jericans. Overall, 62% (n=18) of the soil samples collected from the two sites (Kilifi and Malindi) were positive for larvae which were used as proxy to measure the presence of eggs. The mosquitoes had high preference for human blood (29.81%) and chicken (3.73%) but none had fed on either goat or bovine. Of 259 mosquitoes tested for viral infection, 11.6% were positive forflavivirusonly.ConclusionDomestic and peri-domestic containers were identified to be the key breeding areas of arboviral vectors. Therefore, efforts should be put in place targeting the productive habitat types.