Conditionally Redundant Bacteriocin Targeting byPseudomonas syringae

Abstract

AbstractThe widespread use of antimicrobials under clinical and agricultural settings has resulted in the evolution of resistance to these compounds. To combat the emergence of resistance, current research efforts are focusing on designing treatments to exploit combinations of antimicrobials, where the evolution of resistance confers sensitivity to alternative compounds. In this work we demonstrate that strains ofPseudomonas syringaepossess a natural analogue to this strategy. Specifically, we demonstrate that a single strain produces multiple bacteriocins that can target another strain, but antimicrobial activity of the second bacteriocin is manifest only after resistance to the first emerges. The evolution of resistance also sensitizes the target strain to bacteriocins from a variety of other strains. Strains ofP. syringaetherefore encode multiple bacteriocins that can act in a conditionally redundant manner. It is possible that combinations of bacteriocins could be applied as a cocktail or sequentially, to potentially achieve durable pathogen control.