RESPIRATORY SYNCYTIAL VIRUS NON-STRUCTURAL PROTEIN EXPRESSION ARE LIMITED IN NEUTROPHILS

Abstract

AbstractRespiratory Syncytial Virus (RSV) is a negative stranded RNA virus with a high incidence of secondary bacterial infections. RSV contains two broad immune inhibitory proteins Ns1 and Ns2 which are not present in any other viruses of the Mononegavirales Order. Here we report that expression of Ns2 is attenuated during RSV infection of neutrophils and that RSV is indeed infecting neutrophils rather than simply being phagocytosed by them. Infection was determined by intracellular staining and coinfection studies of uninfected Hep2 cells. The significant attenuation of Ns2 in vivo along with the low abundance of coinfected cells indicates that RSV infection is likely functionally non permissive in in vivo infection. The implications of RSV infection of neutrophils may explain the previously observed phenomenon of decreased phagocytosis in neutrophils exposed to RSV and the lack of Ns2 expression within neutrophils may provide avenues of study to attenuate viral infection through therapy development.