Regional differences in cell characteristics in electric eel electric organs indicate electrocyte development with ventral to dorsal migration

Abstract

AbstractElectric eels (Electrophorus) are renowned for their ability to generate electric discharge, which is used for prey capture and defense. Their electric organs (EOs) are located along the lateral– ventral region of the tail and contain electrocytes, which are multinucleated syncytium cells. Two major hypotheses for the electrocyte origin are proposed: (1) muscle fibers or their precursors, and (2) mesodermal cells not via muscle lineage. In this study, we demonstrate the likely molecular mechanisms and processes involved in this differentiation, supporting the second hypothesis. We report the regional differences in cell morphology within the main EO (mEO) ofElectrophorus. The cell morphology and distribution from the ventral terminal to the dorsal region of the mEO suggest the segregation of progenitors from the ventral cluster and their gradual transformation into mature multinucleated electrocytes via the layering and proliferation stages along the dorsal axis. Myosin-positive muscle cells were not included in the mEO differentiation process. Immunohistochemistry revealed strong expression of sodium-potassium adenosine triphosphatase (Na+/K+-ATPase), a key component in generating electric discharge in the mEO, across most mEO regions, except in the ventral cluster cells. Based on these observations, we propose that electrocyte progenitors develop from ventral cluster cells in the mEO and differentiate into mature multinucleated cells as they migrate dorsally. This is the first report to approach the developmental process ofElectrophoruselectrocytes from cell morphology and genetic profiles. Our findings represent a breakthrough in understanding the differentiation of electrocytes during the growth stages ofElectrophorus.