Regulative synthesis of capsular polysaccharides in the pathogenesis ofStreptococcus suis

Abstract

AbstractStreptococcus suis(S. suis) is an important zoonotic pathogen causing substantial economic losses in the swine industry.S. suisserotype 2 (SS2) is often isolated from the diseased.S. suisexpresses capsular polysaccharide (CPS), a virulence factor crucial for their survival in the blood. However, the role of CPS in the pathogenesis ofS. suisis incomplete. Here, we showed a dynamic regulation of CPS inS. suispathogenesis. In a mouse infection model, an SS2 strain (05ZYH33) was detected in the nasal-associated lymphoid tissue (NALT) and cerebrospinal fluid (CSF) as early as 30 min after intranasal inoculation without bacteremia. Histological analysis revealed that 05ZYH33 in the nasal cavity invaded the olfactory epithelium, resulting in early brain inflammation. Transmission electron microscopy showed that 05ZYH33 isolated from NALT and CSF at early infection time had a thin layer of CPS, and those detected in the blood 5 h post-inoculation showed a much thicker CPS. In addition, adoptive transfer of anti-CPS restricted 05ZYH33 in the blood but not in NALT or CSF. However, an antiserum directed to multiple non-CPS virulence factors (anti-V5) efficiently inhibited 05ZYH33 in NALT, CSF, and blood. Thus, 05ZYH33 colonizes NALT more efficiently without CPS and subsequently invades the meninges through the olfactory nerve system. These findings provide valuable information for the treatment ofS. suisinfection and the development of vaccines across serotypes ofS. suisby targeting CPS-independent immunity.