Differential cytoophidium assembly betweenSaccharomyces cerevisiae andSchizosaccharomyces pombe

Abstract

AbstractThe de novo synthesis of cytidine 5’-triphosphate (CTP) is catalyzed by the enzyme CTP synthase (CTPS), which is known to form cytoophidia across all three domains of life. In this study, we use the budding yeastSaccharomyces cerevisiaeand the fission yeastSchizosaccharomyces pombeas model organisms to compare cytoophidium assembly under external environmental and intracellular CTPS alterations. We observe that under low and high temperature conditions, cytoophidia in fission yeast gradually disassembled, while cytoophidia in budding yeast remain unaffected. The effect of pH changes on cytoophidia maintenance in the two yeast species is different. When cultured in the yeast saturated cultured medium, cytoophidia in fission yeast disassemble, while cytoophidia in budding yeast gradually form. Overexpression of CTPS leads to the presence and maintenance of cytoophidia in both yeast species from the log phase to the stationary phase. In summary, our results demostrate differential cytoophidium assembly betweenSaccharomyces cerevisiaeandSchizosaccharomyces pombe, the two most studied yeast species.