Abstract
AbstractThe Mushroom Body (MB) is a well-characterized associative memory structure within theDrosophilabrain. Although previous studies have analyzed MB connectivity and provided a map of inputs and outputs, a detailed map of the downstream targets is missing. Using the genetic anterograde transsynaptic tracing tool,trans-Tango, we identified divergent projections across the brain and convergent downstream targets of the MB output neurons (MBONs). Our analysis revealed at least three separate targets that receive convergent input from MBONs: other MBONs, the fan shaped body (FSB), and the lateral accessory lobe (LAL). We describe, both anatomically and functionally, a multilayer circuit in which inhibitory and excitatory MBONs converge on the same genetic subset of FSB and LAL neurons. This circuit architecture provides an opportunity for the brain to update information and integrate it with previous experience before executing appropriate behavioral responses.Highlights-The postsynaptic connections of the output neurons of the mushroom body, a structure that integrates environmental cues with associated valence, are mapped usingtrans-Tango.-Mushroom body circuits are highly interconnected with several points of convergence among mushroom body output neurons (MBONs).-The postsynaptic partners of MBONs have divergent projections across the brain and convergent projections to select target neuropils outside the mushroom body important for multimodal integration.-Functional connectivity suggests the presence of multisynaptic pathways that have several layers of integration prior to initiation of an output response.
Publisher
Cold Spring Harbor Laboratory
Reference107 articles.
1. Adden, A.K. , Stewart, T.C. , Webb, B. , and Heinze, S. (2020). A neural model for insect steering applied to olfaction and path integration. bioRxiv.
2. The neuronal architecture of the mushroom body provides a logic for associative learning
3. Aso, Y. , and Rubin, G.M. (2016). Dopaminergic neurons write and update memories with cell-type-specific rules. Elife 5.
4. Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila
5. Bates, A.S. , Schlegel, P. , Roberts, R.J.V. , Drummond, N. , Tamimi, I.F.M. , Turnbull, R. , Zhao, X. , Marin, E.C. , Popovici, P.D. , Dhawan, S. , et al. (2020). Complete Connectomic Reconstruction of Olfactory Projection Neurons in the Fly Brain. Curr Biol.
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