Author:
Mozzachiodi Riccardo,Lechner Hilde A.,Baxter Douglas A.,Byrne John H.
Abstract
The feeding behavior of Aplysia californica can be classically
conditioned using tactile stimulation of the lips as a conditioned stimulus
(CS) and food as an unconditioned stimulus (US). Moreover, several neural
correlates of classical conditioning have been identified. The present study
extended previous work by developing an in vitro analog of classical
conditioning and by investigating pairing-specific changes in neuronal and
synaptic properties. The preparation consisted of the isolated cerebral and
buccal ganglia. Electrical stimulation of a lip nerve (AT4) and a
branch of the esophageal nerve (En2) served as the CS and US,
respectively. Three protocols were used: paired, unpaired, and US alone. Only
the paired protocol produced a significant increase in CS-evoked fictive
feeding. At the cellular level, classical conditioning enhanced the magnitude
of the CS-evoked synaptic input to pattern-initiating neuron B31/32. In
addition, paired training enhanced both the magnitude of the CS-evoked
synaptic input and the CS-evoked spike activity in command-like neuron CBI-2.
The in vitro analog of classical conditioning reproduced all of the cellular
changes that previously were identified following behavioral conditioning and
has led to the identification of several new learning-related neural changes.
In addition, the pairing-specific enhancement of the CS response in CBI-2
indicates that some aspects of associative plasticity may occur at the level
of the cerebral sensory neurons.
Publisher
Cold Spring Harbor Laboratory
Subject
Cellular and Molecular Neuroscience,Cognitive Neuroscience,Neuropsychology and Physiological Psychology
Cited by
29 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献