Author:
Audic Stéphane,Claverie Jean-Michel
Abstract
Genes differentially expressed in different tissues, during development, or during specific pathologies are of foremost interest to both basic and pharmaceutical research. “Transcript profiles” or “digital Northerns” are generated routinely by partially sequencing thousands of randomly selected clones from relevant cDNA libraries. Differentially expressed genes can then be detected from variations in the counts of their cognate sequence tags. Here we present the first systematic study on the influence of random fluctuations and sampling size on the reliability of this kind of data. We establish a rigorous significance test and demonstrate its use on publicly available transcript profiles. The theory links the threshold of selection of putatively regulated genes (e.g., the number of pharmaceutical leads) to the fraction of false positive clones one is willing to risk. Our results delineate more precisely and extend the limits within which digital Northern data can be used.
Publisher
Cold Spring Harbor Laboratory
Subject
Genetics (clinical),Genetics
Reference32 articles.
1. Toward the development of a gene index to the human genome: an assessment of the nature of high-throughput EST sequence data.
2. Adams M.D. (1996) Progress towards a complete set of human genes. in Genomes, molecular biology and drug discovery, eds Browne M.J. Thurby P.L. (Academic Press, London, UK).
3. Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project
4. Sequence identification of 2,375 human brain genes
5. Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence. (The Genome Directory, Suppl.);Adams;Nature,1995
Cited by
2501 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献