Genetic controls of mouse Tas1r3-independent sucrose intake

Author:

Lin Cailu,Tordoff Michael G.,Li Xia,Bosak Natalia P.,Inoue Masashi,Ishiwatari Yutaka,Beauchamp Gary K.,Bachmanov Alexander A.,Reed Danielle R.

Abstract

AbstractWe have previously shown that variation in sucrose intake among inbred mouse strains is due in part to polymorphisms in the Tas1r3 gene, which encodes a sweet taste receptor subunit and accounts for the Sac locus on distal Chr4. To discover other quantitative trait loci (QTLs) influencing sucrose intake, voluntary daily sucrose intake was measured in an F2 intercross with the Sac locus fixed; in backcross, reciprocal consomic strains; and in single- and double-congenic strains. Chromosome mapping identified Scon3, located on Chr9, and epistasis of Scon3 with Scon4 on Chr1. Mice with different combinations of Scon3 and Scon4 genotypes differed more than threefold in sucrose intake. To understand how these two QTLs influenced sucrose intake, we measured resting metabolism, glucose and insulin tolerance, and peripheral taste responsiveness in congenic mice. We found that the combinations of Scon3 and Scon4 genotypes influenced thermogenesis and the oxidation of fat and carbohydrate. Results of glucose and insulin tolerance tests, peripheral taste tests, and gustatory nerve recordings ruled out plasma glucose homoeostasis and peripheral taste sensitivity as major contributors to the differences in voluntary sucrose consumption. Our results provide evidence that these two novel QTLs influence mouse-to-mouse variation in sucrose intake and that both likely act through a common postoral mechanism.

Publisher

Cold Spring Harbor Laboratory

Reference79 articles.

1. Postnatal growth velocity modulates alterations of proteins involved in metabolism and neuronal plasticity in neonatal hypothalamus in rats born with intrauterine growth restriction

2. Transplantation genetics: current status and prospects;Am J Transplant,2014

3. Anonymous (2011). Mouse Genomes Project - Query SNPs, indels or SVs. Wellcome Trust Sanger Institute.

4. Anonymous. (2012). “SIFT web server: predicting effects of amino acid substitutions on proteins.” August 15th 2015. Retrieved 10/7/2016, 2016, from http://siftdna.org/www/SIFT_dbSNP.html.

5. Anonymous (2015). Mus musculus (laboratory mouse) genome view, National Center for Biotechnology Information.

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