Pantr2, a trans-acting lncRNA, modulates the differentiation potential of neural progenitors in vivo

Abstract

Ablation of the long non-coding RNA (lncRNA) Pantr2 results in microcephaly in a knockout murine model of corticogenesis, however, the precise mechanisms used are unknown. We present evidence that Pantr2 is a trans-acting lncRNA that regulates gene expression and chromatin accessibility both in vivo and in vitro. We demonstrate that ectopic expression of Pantr2 in a neuroblastoma cell line alters gene expression under differentiating conditions, and that both loss and gain of function of Pantr2 results in changes to cell-cycle dynamics. We show that expression of both the transcription factor Nfix and the cell cycle regulator Rgcc are negatively regulated by Pantr2. Using RNA binding protein motif analysis and existing CLIP-seq data, we annotate potential HuR and QKI binding sites on Pantr2, and demonstrate that HuR does not directly bind Pantr2 using RNA immunoprecipitation assay. Finally, using Gene Ontology enrichment analysis, we identify disruption of both Notch and Wnt signaling following loss of Pantr2 expression, indicating potential Pantr2-dependent regulation of these pathways.