INCREASING DIVERSITY AMONGLENSSPECIES FOR IMPROVING BIOLOGICAL NITROGEN FIXATION IN LENTIL

Abstract

ABSTRACTExotic germplasm is a key resource for reintroducing genetic variability into cultivars. We evaluated 36 accessions from cultivated lentil (Lens culinarisMedik.) and six related wild species, inoculated with a commercial strain ofRhizobium leguminosarumbv.viciaeunder greenhouse conditions. The objective was to exploreLensspecies and/or accessions that could contribute higher biological nitrogen fixation (BNF) ability to the lentil crop. A split plot design was used with eitherRhizobiuminoculation, added Nitrogen (N) or neither, as the main plots, and accessions in subplots randomized in blocks. Two repeats of the experiment were evaluated at flowering for N fixation and nodulation characters, and two subsequent experiments, with a subset of 14 accessions, were evaluated at maturity for seed production, seed quality and harvest index. Differences in phenotypic expression did not correspond to any particularLensspecies. CDC Greenstar exhibited some of the highest N fixation values observed among the cultivars and also superior yield results compared to the added N treatment. Wild accessions, including IG 72643 (L. orientalis), displayed unique and multiple desirable characteristics compared to cultivars including indeterminate nodulation, higher N translocation, stable yield compared to added N treatment and exceptionally high protein concentration in seeds.