An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation inC. elegans

Abstract

AbstractThe auxin-inducible degradation system has been widely adopted in theC. elegansresearch community for its ability to empirically control the spatiotemporal expression of target proteins. This system can efficiently degradeauxin-inducibledegron (AID)-tagged proteins via the expression of a ligand-activatableAtTIR1 protein derived fromA. thalianathat adapts target proteins to the endogenousC. elegansproteosome. While broad expression ofAtTIR1 using strong, ubiquitous promoters can lead to rapid degradation of AID-tagged proteins, cell type-specific expression ofAtTIR1 using spatially restricted promoters often results in less efficient target protein degradation. To circumvent this limitation, we have developed a FLP/FRT3-based system that functions to reanimate a dormant, high-powered promoter that can drive sufficientAtTIR1expression in a cell type-specific manner. We benchmark the utility of this system by generating a number of tissue specific FLP-ON::TIR1 drivers to reveal genetically separable cell type-specific phenotypes for several target proteins. We also demonstrate that the FLP-ON::TIR1 system is compatible with enhanced degron epitopes. Finally, we provide an expandable toolkit utilizing the basic FLP-ON::TIR1 system that can be adapted to drive optimizedAtTIR1expression in any tissue or cell type of interest.