Role of DEAD Box RNA helicases in low temperature adapted growth of AntarcticPseudomonas syringaeLz4W

Abstract

AbstractNucleic acid metabolism plays an important role in adaptation of organisms to environment. For adaptation to cold environment, organisms need to overcome the impediments in complex RNA secondary structures that are highly stabilized at low temperature affecting metabolism and growth of the organisms. Employing gene disruption through homologous recombination, we investigated the role of all major DEAD box RNA helicases (rhlE, srmB, csdA, dbpAandrhlB) in cold adapted growth ofPseudomonas syringae. Our findings concluded thatsrmBanddbpAgenes are important for cold adapted growth, whereascsdAis indispensable for growth at low temperature. Helicase genesrhlBandrhlEhave no appreciable role in cold adapted growth ofPseudomonas syringae. Functional complementation studies revealed that RNA helicases don’t have any redundant functions, as the roles performed by different helicases are individual and specific.