Author:
Sylvain François-Étienne,Leroux Nicolas,Normandeau Eric,Custodio Jaqueline,Mercier Pierre-Luc,Bouslama Sidki,Holland Aleicia,Barroso Danilo,Val Adalberto Luis,Derome Nicolas
Abstract
AbstractAmazonian blackwaters are extremely biodiverse systems containing some of the most naturally acidic, dissolved organic carbon-rich and ion-poor waters on Earth. Physiological adaptations of fish facing these ionoregulatory challenges are unresolved but could involve microbially-mediated processes. Here, we characterize the physiological response of 964 fish-microbe systems from four blackwater Teleost species along a natural hydrochemical gradient, using dual RNA-Seq and 16S rRNA of gill samples. We find that responses to blackwaters are host-species-specific, but occasionally include the overexpression of Toll-receptors and integrins associated to interkingdom communication. Blackwater gill microbiomes are characterized by a transcriptionally-active betaproteobacterial cluster potentially interfering with epithelial permeability. We explore further blackwater fish-microbe interactions by analyzing transcriptomes of 320 axenic zebrafish larvae exposed to sterile, non-sterile and inverted (non-native bacterioplankton) blackwater. We find that axenic zebrafish do not survive well when exposed to sterile/inverted blackwater, suggesting an essential role of endogenous symbionts in blackwater fish physiology.
Publisher
Cold Spring Harbor Laboratory
Reference132 articles.
1. The influence of dissolved organic matter (DOM) on sodium regulation and nitrogenous waste excretion in the zebrafish (Danio rerio);Jxournal of Experimental Biology,2016
2. Lactobacillus plantarum MB452 enhances the function of the intestinal barrier by increasing the expression levels of genes involved in tight junction formation
3. Triportheus albus Cope, 1872 in the Blackwater, Clearwater, and Whitewater of the Amazon: A Case of Phenotypic Plasticity?
4. The bacterial signal indole increases epithelial-cell tight-junction resistance and attenuates indicators of inflammation
5. Beaumont M , Neyrinck AM , Olivares M , Rodriguez J , de Rocca Serra A , Roumain M , et al., The gut microbiota metabolite indole alleviates liver inflammation in mice. FASEB J.2018:fj201800544.